Streptococcus pyogenes is a major human pathogen that causes several infections. Recently, it has become harder to control because it has become more resistant to common antibiotics. This fact has made people more interested in locating new natural sources of compounds that kill bacteria. Soil bacteria are a big part of modern microbial research because they can make bioactive secondary extracts. The goal of this study was to find and test the antimicrobial properties of several soil sours , with a focus on Streptococcus pyogenes, and to connect the chemical makeup of the bacterial extracts to their biological activity. We took soil samples from different places and used standard lab methods to isolate and purify bacterial strains. We used three common bioassay methods cross-line, disk diffusion, and beaker agar to test how well the antibacterial activity worked. There were clear differences between the isolates in terms of how well they inhibited growth. One isolate had higher and more stable activity than the others. High-resolution mass spectrometry (HRMS) analysis of the most potent extract showed that it contained several organic compounds from different chemical classes, such as lipids and heterocyclic compounds. These compounds are likely to help the extract fight bacteria. These findings suggest that soil bacteria could be a promising way to identify new natural compounds that could help make new treatments for the growing problem of bacterial resistance (Cunningham, 2000; Ventola, 2015; Bardi, 2012).